By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. Detergent Compatible Bradford assay. #N207) LAL Reagent Water (Lonza cat. Fluorescence Microplate Reader Comparison Chart. The VersaMax Absorbance Microplate Reader measures the optical density (OD) of samples at selected wavelengths in a number of reading modes. The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. Standards, controls and blanks were pipetted into the wells and incubated followed by washing with the Molecular Devices MultiWash™+ EMax Plus R2 = 0. 96-well microplate: 90 sec; White light: 96-well/ 384 well microplate: <2 min. 03 pM. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader. Description. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. 3. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. Paradigm: Delta mP. < 0. Plates can put in the drawer both in landscape and portrait positions. Once the assay runs its course, the final absorbance readout will take place on a SpectraMax® iD5 Multi-Mode Microplate Reader. Molecular Devices microplate readers have the most variety of tools available for users to meet their diverse hardware and software validation needs. Deep well blocks available in 24, 96 and 384-well formats Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. Also refer to this Multimode Reader Comparison Chart. I contacted Molecular Devices and they quoted me almost $6k to come out and replace. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. 125 pM * Specifications listed are for europium 96-well: 0. The VersaMax and SpectraMax 190 read 96-well microplates. The top- and bottom-reading Gemini EM Microplate Reader with dual monochromators lets users determine the optimal excitation and emission settings for creative new fluorescence intensity assays. Wavelength Range. 030 fmol Fluorescence Polarization (FP). 7°C and the bottom to 36. Reactions catalyzed by firefly and *Renilla luciferases. #3693; n = 4 for each standard). 375 pM. Solid black plates usually give the least fluorescent background noise, allowing better sensitivity. The procedure enables direct translation of any published equation. The microplate washer automatically washes all wells of the assay plate, using the desired wash program to completely remove unbound reagents and eliminate background noise. Simple, economical . VANTAstar™ Flexible Multi-mode Microplate Reader. No additional manuals are available. and luminescence microplate reader validation DATA SHEET. Reads standard cuvettes & 96-384 well plates from 190 to 1000 nm. 003 fmol 0. 2 OD at 200 nm; this decreased to 0. SpectraDrop Micro-Volume Refills, 24-Well Bottom Slides. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. When the user applies a set temperature of 37°C, the top is heated to 37. section, select plate section titled. 001 OD Read modes (EMax): Endpoint Read modes (VMax): Endpoint, kinetic Read times (96-well microplate): Endpoint read-only: 5 seconds. Instrument settings are listed in Table 1. With various on-board features and. > Molecular Devices LLC > microplate reader. The plate reader optics are tunable for. Equipped with scanning, shaking and kinetics modules. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. SpectraMax iD3 Multi-Mode Microplate Reader (Molecular Devices cat. #G7294). The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurements in the. Microplate spectrophotometers, also called microplate readers, allow researchers to analyze multiple samples in parallel using 6- to 1536-well formats, as opposed to single sample measurements. This richly featured reader gives you the speed, convenience, and economy of monochromator-based wavelength selection for visible absorbance measurements between 340 nm and 850. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M4 Microplate Reader transforms each well in a microplate to a fixed. Place the microplate in the drawer. 0 OD Photometric resolution: 0. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). Keywords: fluorescence polarization, trf, fret, igg. Reading a Microplate . They are widely used in research, drug discovery, [1] bioassay validation, quality control and manufacturing processes in the pharmaceutical and. Taking into account the price differences between each seller and each country. 2 OD at 200 nm; this decreased to 0. U-bottom: 30-40 µl. 0 500 1000 1500 2000 2500 3000 3500 time (sec. the assay. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Read More. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories. for 96- and 384-well microplates • Detect dim samples with unsurpassed sensitivity • Avoid saturation issues with nine orders of dynamic range • Enable robust performance with autorinse injectors • Validation and compliance tools SpectraMax® L Microplate . A single-mode plate reader is a good starting point. ) 20,000 10,000 30,000 40,000 50,000 60,000 70,000 80,000 0. Optical System: High energy xenon flash lamp. The CellTiter-Glo assay from Promega uses luciferase. $489. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. The SpectraMax® ABS Plus Microplate Reader can run . #L6876) • Guanidine hydrochloride (Sigma cat. a white 96-well half-area microplate (Corning cat. The EMax Plus reader measures flat and round 96-well microplates. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers Molecular Devices LLC Applications: Absorbance, Cell Health, Cell Viability, Cellular. #3610) BrightMax sealing films (Genesee cat. #12-639) White 96- and 384-well microplates (Greiner cat. Contents of wells were pipetted up and down several times to mix. “The ABS Plus reader comes standard with our patented PathCheck Technology which enables consistent results across a 96- or 384-well plate. The ThermoMax performs automatic mixing before a reading and between readings to ensure reliable and precise. SpectraMax 250 User. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. Standard features on the EMax® Plus Microplate Reader include the ability to measure both flat and round 96-well microplates, automatic lamp calibration prior to each reading (ensuring automatic measurements), powerful curve fitting protocols and statistical analysis tools, and integrated instrument control and data analysis with SoftMax® Pro. Pipet 100-200 µL of deionized water into each well of a clean 96-well microplate. The direct transfer capability reduces reagent consumption and allows for more assay conditions to be explored in a single microplate, making the system more amenable to agonist and antagonist assay formats. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. 1536-well: 0. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. 96-well microplate: 90 sec; White light: 96-well/ 384 well microplate: <2 min. Absorbance was read at 480 nm on the SpectraMax ABS Plus reader. The system provides automation for up to 50 microplates for easy walkaway automation. The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . white 96 well plate and 75. Type: Microplate Reader. validation, maintenance, and repair documentation. The instruments offer 96- and 384-well microplate-to-microplate fluid transfer (8 or 16 wells at a time), and can read microplates having 6, 12, 24, 48, 96, and 384-well formats. All stand-alone instrument functions can be controlled using the software. FlexStation 3 Benchtop Multi-Mode Microplate Reader User Guide Ordering Parts and Accessories To obtain parts and accessories, contact Molecular Devices Customer Service or your authorized service representative. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. 46Click the Read button in the SoftMax Proplated at 50,000 cells/well in 100 µL of DMEM/FBS in a 96-well plate and incubated at 37°C for five hours. Multi-Mode Reader with the StakMax Microplate Handling System from Molecular Devices. book Page 1 Wednesday, October 12, 2011 4:56 PMBuilt around a grating monochromator, the VersaMax™ Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340–850nm). The SpectraMax® i3x Multi-Mode microplate reader measures spectral- based Absorbance, Fluorescence, and Luminescence on microplates that range from 6 to 384 wells. explore more. Microscopy. Protein quantitation Cross-talk < 0. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. Upgradeable modules are also available including western blot, cell imaging, and fast kinetics with injectors. Highly customizable low volume applications Molecular Devices unique SpectraDrop Wide photometric range Temperature-regulated Thermal control and vertical shake Microsoft Windows-based software Automatic mixing Five-second read per plate Photometric and kinetic analysis mode The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. As a result, assay flexibility is consequence-free. A control well was set up to verify. Ordering information FlexStation 3 Microplate Reader Part Number: FLEX3 • FlexStation 3 Base System Consumables 96-Well, FlexStation Pipet Tips (Black) Part Number: 9000-0911 • 200 µL capacity • Includes 3 vials each of FLIPR Calcium 6 and 6-QF dyes Reagents FLIPR® Calcium 6 Assay Evaluation Kit Part Number. Multi-Mode Microplate Reader for . Industry standard, 96-well plates are. 5. In combination with the SpectraMax Mini Multi-Mode Microplate Reader, it enables increased throughput and detection of DNA as low as 2 ng/μL using just a few microliters of sample. Page 1 ™ VERSAmax Tunable Microplate Reader Operator’s Manual for Instruments with Serial Numbers Beginning with BN Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0084 Rev. All stand-alone instrument functions can be controlled using the software. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. ©2017 Molecular Devices, LLC . July 17, 2023. F3/F5 Multi-Mode Microplate Readers. Fax 4200 BERTHOLD TECHNOLOGIES Lumat LB 9507 Tecan SpectraFluor Thermo Scientific Varioskan LUX LJL Biosystems Analyst AD 96. This is equivalent to having 511 built-in filters, Polypropylene Deep well Plates are used as small test tubes because of easier sealing, flat or conical shape, square and round well formats with lids and cap. Temperature Control: Ambient +5C up to 42C. Devices® SPECTRAmax 190 microplate reader at a wavelength of 600 nm. The SpectraMax iD3 reader is a stand-alone multi-mode microplate reader that introduces NFC based user authentication and touchscreen interface personalization. 6:30 . Our SpectraTest® ABS2, FL1, LM1,The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. 1700 Online: Sales Offices. 3 OD at 200 nm. firefly luciferase in 96- and 384-well top read Dynamic range: > 6 decades Cross-talk: < 0. 40266. Molecular. 10 µL of BSA standard or cell lysate was pipetted into wells of a 96-well microplate (n = 3). #R8340) SpectraMax i3x Multi-Mode Microplate Reader (Molecular Devices cat. Video Description: The video below gives an overview of the Molecular Devices SpectraMax M2 Multi-Mode Microplate Reader, and also demonstrates its functionality. Compact design Minimum volume and maximum throughput with 96- or 384-well microplate compatibility Powerful data analysis with SoftMax® Pro Software Optimal performance with validation tools Small but Mighty The IC design and microplate construct represents a major advancement to state-of-the-art CMOS devices for biological measurement, where the large majority are formed in single well constructs 12. personalized as your research. 96-well plates: V-bottom: 20-30 µl U-bottom: 30-40 µl flat-bottom: 50-60 µl 384-well plates: approximately 20 µl Recommended ArticlesA method is described for the routine determination of the rate of colorimetric enzyme reactions using a 96-well microtiter plate reader commonly used in. With various on-board features and capabilities,. 96-well solid bottom, clear polystyrene microplates (Greiner cat. microplate . Microplate Capacity Standard 96-well microplates Incubation Temperature control up to 50ºC Temperature uniformity ± 0. The reader can measure flat and round 96-well microplates and ensure accuracy by automatic lamp calibration before each reading. An industry-renowned instrument for monitoring GPCRs and ion channels. 04 sec. 63. SoftMax Pro Software allows you to set up and run a complete protocol for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. 54: 1. For more sample throughput, both readers can be easily integrated into full roboticPrimary considerations when choosing a microplate reader are throughput and flexibility. Its software interface is easy to learn and use, and lets you control the instrument from almost any device –. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. 2. Workflows of the MTT Cell Proliferation and Cell Cytotoxicity Assay. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. CloneMatrix. g. Other Molecular Devices readers with absorbance detection mode, e. Microplate is validated by molecular devices for the FLIPR reader. The AquaMax® Microplate Washer is a fully self-contained system, configurable for both 96- and 384-well microplates. Step 1: Turn on your microplate reader. The clear UltraVision FC 96-well microplate (FC_UltraVision (clear, 96)) showed the best performance of the tested microplates, with absorbance of 0. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid-Molecular Devices Tunable Microplate Reader VersaMax $ 1,495. Brand: MOLECULAR DEVICES. 0 96 Well Plate. Type:Single Mode Absorbance Plate Formats:96 well plates Accuracy:0. Paradigm: Delta mP. Microplate Readers. Products : General, FilterMax F5 reader. #R8340) SpectraMax i3x Multi-Mode Microplate Reader (Molecular Devices cat. 3% in white 96- and 384-well microplates Time-Resolved Fluorescence Performance (M4, M5, M5e only) Reading capabilities Top or bottom of a microplatethe iD5 reader’s filter system. None for any other plate (6, 12, 24, 48 well plates) Flex3: Both Top read and. #i3x) SpectraMax L Microplate Reader (Molecular Devices cat. Fastest Read Time - 96-well plate: 20 sec; 384-well plate: 30 sec. keeping with best practices, reader performance should still be validated and documented regularly, especially in regulated laboratories. Published Dec 16, 2020 | Updated Dec 16, 2020. Category 2 and 3 microplate readers use gradient heating as well. Go to Product Details. Pipet 100-200 µL of deionized water into each well of a clean 96-well microplate. For fluorescence intensity assays, users can select from top- or bottom-read modes for improved sensitivity in solution and cell-based assays. 69: 85. the SpectraMax i3x Multi-Mode Microplate Reader, may also be used for this application. The reader measures both flat and round 96-well microplates and ensures accuracy by automatic lamp calibration prior to. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). The drawing shows a standard microplate of 15 mm height covered with a lid. 3% in white 96- and 384-well microplates Time-Resolved Fluorescence Performance (M4, M5, M5e only) Reading capabilities Top or bottom of a microplateWide photometric range Temperature-regulated Thermal control and vertical shake Microsoft Windows-based software Automatic mixing Five-second read per plate Photometric and kinetic analysis mode The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. DNA concentration (ng/mL) Average RFU. We have been using this for DNA measurements 260/280 nm, colorimetric assay measurements like MTT assays, and ELISAs. 10 µL of BSA standard or cell lysate was pipetted into wells of a 96-well microplate (n = 3). It also allows for the elimination of standard curves. 384 Awareness Technology ChroMate BioTek Synergy Mx. 2 W x 44. All stand-alone instrument functions can be controlled using the software. 96-well: 0. Optimized for fluorescence microplate readers. Resipher 32x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 32x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 32 wells Utilizes Lucid Lab Cloud software for connection to any…. The reader measures both flat and round 96-well microplates and ensures accuracy by automatic lamp calibration prior to. Instrument settings can be saved as a protocol file and used repeatedly for reading differentThe Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. The list is a standard grouping supplied by Molecular Devices and includes some which cannot be used on this instrument; they can be deleted. The spectrophotometer has a wide wavelength range 190. The SpectraDrop Microplate’s slide design eliminates the need for calibration and gives consistent well-to-well reads with CVs below 5%. Wavelength Range 200 to 999 nm. At 5 seconds to read a 96-well endpoint assay or a 5 second minimum interval between kinetic reads, the VMax Microplate Reader has both speed and performance. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical pathlength) of samples with no temperature. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. The QuickSync client and automatic USB export option not only frees the user from waiting for data in front of the plate reader, but automatically sends it to one’s computer in an. Prior to the reading of each plate, the microplate reader was “blanked” with a plate containing 0. Microplate readers read biological, chemical or physical events that occur on a microtiter plate. 92: 88. 96-well microplate; Immunoassay analyzer;VersaMax price. 4% in white 96- and 384-well microplates Sensitivity (ATP-Glow) Optimized 96 wells 3 pM 384 wells 6 pM * With 6 flashes in absorbance and 3 flashes in fluorescence mode and 0. Software Validation. Operates as a stand-alone reader or with input/output stackers and bottom reading optics. Technical specifications Performance specifications Cross-talk < 3 x 10-5 (96-well) Read modesThe SpectraMax® i3x Multi-Mode Microplate Reader measures spectral-based . Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. # 651101) the iD5 reader’s filter system. SpectraMax® Microplate Reader Suite of Products Knowledge Base. An enzyme-linked polyclonal antibody specific for mouse/rat IL-22 was added to the wells and. White 96-well plate The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Page 11: Reader ComponentsBioTek Synergy HTX Microplate Reader - Absorbance Only Specifications. Vrla used the device to quantify the concentration of a small molecule produced by. We would like to show you a description here but the site won’t allow us. The FilterMax F5 can read 6, 12, 24, 48, 96, 384, 1536-well. Molecular Devices Corporation (the “Seller”) shall not be liable for any incidental or consequential damages. Absorbance was read at 480 nm on the SpectraMax ABS Plus reader. (96, 384, or 1536 wells) and the "Select Specific". best-in-class data . University of Reading Simon Lydford: Molecular Devices (UK) Ltd. buffers for (2) 96-well microplates R8088 CatchPoint cAMP 96-well Fluorescent Assay Kit (Bulk) ELISA-based assay for the detection and quantification of cAMP * Contains microplates, reagents, and buffers for (10) 96-well microplates R8089 SpectraMax® i3/i3x Multi-Mode Microplate Reader SpectraMax® Paradigm® FlexStation® 3 Multi-Mode. Standard SBS 96 and 384 well plates (128. 2600A October 2023. If read values are low, try setting PMT sensitivity to High in the Settings menu of the Plate section. The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. Microplate Formats: 6, 12, 24, 48, 96, 384. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories involved in many applications. Triplicates of each BSA concentration were made for this assay plate. 96-well and 384-well plates; Wavelength(s): 405, 450, 490, 560 and 600nm (Abs) Specifications/Features: Shaking, Temperature control, Injector. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Molecular Devices SpectraMax 384 Plus Microplate Reader. Blank wells containing 50 µL culture medium only (n = 8) were also prepared. • SpectraMax® Plus 384 Microplate Reader (Molecular Devices cat. SmartReader 96: Designed for optimal ELISA Testing. VersaMax Microplate Reader An affordable, tunable microplate reader for 96-well format Affordable The robot-compatible VersaMax reader is cost-effective—users never have to buy filters. The SpectraMax M4 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96 and 384-well microplates. The onboard luminescence detection mode may be used, or, for higher sensitivity, the reader can be equipped with a NanoBRET detection cartridge that offers a 4-fold improvement in limit of quantitation. Growth medium was then replaced with fatty acid uptake solution. SpectraMax iD5 Multi-Mode Microplate Reader (Molecular Devices cat. Fluorescence Microplate Readers. Avoid losing high signal. 03 pM 384-well: 0. The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. Never order or replace filters to optimize an assay again!microplate readers from Molecular Devices. #iD5-STD) Methods ATP standard curve. However, an upgradeable multi-mode reader which includes absorbance, fluorescence and luminescence can address all. With theThe StakMax® Microplate Handling System is a powerful, compact, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers for improved process flow and higher throughput. NOTE: Limited support and literature is available for the SpectraMax 250 due to the senescence of the product. In labs where throughput is a top priority, a device that can screen 96- to 1536-well microplates in under one minute is worthwhile. Compare specifications and request a quote from leading suppliers. The SpectraMax ABS Plus Microplate Reader can run both cuvette-based and microplate based reader applications on the same instrument. FlexStation® 3 Benchtop Multi-Mode Microplate Reader combines Molecular Devices’ SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact benchtop reader. Compact design Minimum volume and. San Jose, California, June 5, 2018. Molecular Devices announced today the launch of the new SpectraMax ® ABS and ABS Plus Microplate Readers that cover a wide range of absorbance-based applications such as ELISAs, microbial growth, and protein quantitation and can be easily integrated into full robotic systems. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. SpectraMax® Plus 384 Microplate Reader (Molecular Devices cat. Despite its form, the Absorbance 96 delivers precise and accurate results at an affordable price. Detect dim samples with unsurpassed sensitivity; Avoid saturation issues with nine orders of dynamic range; Enable robust performance with injectors; Validation and compliance tools; Learn moreThe Molecular Devices microplate reader portfolio comprises various instruments, which differ regarding the design of the heating elements, as well as temperature control, and are therefore divided into three categories as shown in Table 1. In addition, SoftMax Pro SoftwareUsing Molecular Devices microplate readers and SoftMax. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. When reading at wavelengths. absorbance, fluorescence, and luminescence with the added functionality of. research hardware on the International Space Station, providing researchers the ability to conduct microplate reader experiments in microgravity for the first time. Description. A. 340‚ 850 nm. Read one sample or up to 384 in a single plate eead using any standard cuvette, or 96-or 384-well microplate. Wren supernatant was transferred to a fresh 96-well microplate and absorbance recorded at 620 nm using a SpectraMax M3 (Molecular Devices) spectrophotometer. Measure more accurately. ***Figure 1. #L6876) Guanidine hydrochloride (Sigma cat. Wavelength (s) 200 to 1000 nm (Abs); 250 to 850 nm (Fluor); 250 to 850 nm (TRF) • The FlexStation™ II³⁸⁴ instrument is designed for 96- and 384-well microplates. microplates or in a cuvette at a. You can add your own protocols. “The ABS Plus reader comes standard with our patented PathCheck Technology which enables consistent results across a 96- or 384-well plate despite volume. Tecan Infinite 200 Pro M Plex Microplate Reader Unit2 - AV. SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. Infinite 200 Pro Microplate Reader. 0. 384 well polystyrene microplates have plate dimensions of 127. Limit of Detection: < 20 attomol ATP per well Dynamic range > 9 orders of magnitude. to each well. Instrument settings can beAbsorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. Outer wells are aligned for proper viewing with FLIPR. #N207) LAL Reagent Water (Lonza cat. The color touch screen provides a visual user interface, making programming fast and intuitive. Cells were washed three times with calcium wash buffer using an AquaMax 4000 Microplate Washer with 96- or 384-well cell wash head. The FlexStation® 3 Multi-Mode Microplate Reader can be used to run the SpectraMax DuoLuc assay in 96- and 384-well formats with high sensitivity and throughput. 0 mm Holex HEXAGON ballhead bolt driver to loosen the screw located inside the Transport Lock opening until you feel the spring release. The contents of the SpectraDrop Microplate kit depend on kit type. Mix well by trituration or plate shaker and incubate for 2 to 5 minutes at room temperature, protected from light. Fluorescence Microplate Reader Comparison Chart. Both readers were heated to 37ºC prior to setting up the assay. 14: Average %CV. 0. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. Since then, the microplate measurements of DNA, RNA, and proteins that this enabled have become very popular. We obtained three HEK-293 cell lines from Clontech, each stably transfected with a different fluorescent protein. , Sigma cat. Page 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. 5°C at 37°C. AquaMax 4000 Microplate Washer (Molecular Devices Cat. Microplate readers use a vertical light path so the distance of the. The system is operated through the industry-leading SoftMax® Pro. The instruments offer 96- and 384-well microplate-to-microplate fluid transfer (8 or 16 wells at a time), and can read microplates having 6, 12, 24, 48, 96, and 384-well3. SoftMax® Pro Microplate Data Acquisition and Analysis Software Version 6 User Guide 5014177 C October 2011 FrameMaker_Export. The reduced cost of the device represents the major technical contribution of this manuscript, as costs were reduced 7 fold from previous reports. It is user upgradeable and budget friendly, and comes with a brochure that provides detailed information on its features, specifications and applications. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. 96-well: 0. 96- or 384-well microplate compatibility. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. 92: 88. Microplate readers compatible with 96-well plates Single- to multi-mode readers combine an absorbance plate reader , a luminescence plate reader , and a fluorescence plate. Up to 70% savings in overall assay time can be gained when using the Cell Cytotoxicity assay (Figure 1). The FilterMax™ F3 features absorbance (visible), fluorescence intensity - FI (top reading), and glow luminescence for 96- to 384-well plates. 1 mL/well of media. A microplate reader as . 3:40 . #PLUS 384) Methods. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical. The SpectraMax Mini Multi-Mode Microplate Reader is a compact and versatile instrument that can measure absorbance, fluorescence and luminescence in microplates. Molecular Devices VersaMax Microplate Reader Specifications. Condition: Used - Good. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Molecular Devices gmbH Tel. Pick the best speed, sensitivity, and price for your needs. Resipher 96x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 96x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 96 wells Utilizes Lucid Lab Cloud software for connection to any…. Lucid Scientific. Plate type 96-well standard clear bottom: Timing Total run time: 01:40:00: Interval: 00:01:00 More settings : Carriage speed: Slow:The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Wavelength Selection. Open the catalog to page 5. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. Other features and benefits include: Eliminate changing out filters. Biochemical characterization was conducted, using GEN III MicroPlate™ Biolog identification system and confirmed with molecular identification via 16S rDNA gene. Plate readers, also known as microplate readers or microplate photometers, are instruments which are used to detect biological, chemical or physical events of samples in microtiter plates. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. Bioz Stars score: 86/100, based on 1 PubMed citations. 3. Description. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. 5. Efficient cell washing with minimal cell loss and optimal assay performance. ©2017 Molecular Devices, LLC 3/17 0120-1441I Printed in USA Figure 3. Bioz Stars score: 86/100, based on 1 PubMed citations.